# Mechanism of Enzyme Action

Mechanism of enzyme action are as follows:

1. Lock and key model:

$\underset{\text{Enzyme catalyst (key)}}{E} + \underset{\text{Substrate (Lock)}}{S} \leftrightharpoons \underset{\text{Enzyme substrate complex}}{E- S} \to \underset{\text{Enzyme}}{E} + \underset{\text{Product}}{P}$

2. Kinetics of enzyme catalysis: According to Michaelis-Menten the enzyme catalysed reaction occurs as follows:

$E(Enzyme) + S \leftrightharpoons ES(Enzyme substrate complex) \underset{fast}{\rightarrow} EP \overset{K_2}{\rightarrow} E + P(Product)$

$H_2 NCONH_2 (Urea) H_2 O \overset{Urease}{\rightarrow} 2NH_3 + CO_2 \uparrow$

$C_6H_{12}O_6 + H_2O \overset{Zymase}{\rightarrow} 2C_2H_5OH + 2CO_2 \uparrow$

Kinetics of enzyme catalysis

$2H_2O_2 \overset{Catalase}{\rightarrow} 2H_2O +O_2$

$C_2H_5OH + O_2 \overset{Microderm}{\underset{acetei} \rightarrow} CH_3COOH + H_2O$

The origin of catalase is blood, plant juice etc.

• Lactic bacili present in curd catalyzes the fermentation of milk. The enzyme lipase catalyzes the fermentation of sugar to produce glycerol.
• Digestion of food is a very complicated process. But enzymes such as pepsin and ptyalin (origin—saliva) in gastric juice work together to increase the rate of digestion i.e.to break down larger molecules of proteins and starch into smaller molecules which may be easily utilized by the cells of the body. Evidently, enzymes are very important in controlling the biological processes.

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